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Volume 22 , Issue 6
November/December 2002
Pages 547–557

Technique for Producing Platelet-Rich Plasma and Platelet Concentrate: Background and Process

Aron Gonshor, BSc, PhD, DDS, FRCD(C)

PMID: 12516826
DOI: 10.11607/prd.00.0495

Autologous platelet-rich plasma is a source of platelet-derived growth factor (PDGF) and transforming growth factor beta (TGF-
), both important in accelerating hard and soft tissue maturation. This article describes a two-step centrifugation method to sequester and concentrate platelets to a level four to eight times baseline whole blood values. The technique produces concentrations of PDGFAB above 500% and TGF-
1 greater than 800%. Flow cytometry measuring pselectin expression shows that the platelets remain quiescent throughout the procedure, maintaining their integrity and viability, with no inadvertent activation. (Int J Periodontics Restorative Dent 2002;22:547–557.)

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